Changes in the expression of MDR transporters in J774 mouse macrophages selected by chronic exposure to ciprofloxacin (CIP) or moxifloxacin (MXF), two fluoroquinolone antibiotics
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چکیده
C. Vallet, B. Marquez, N. Nhiri*, E. Jacquet*, M-P. Mingeot-Leclercq, P.M. Tulkens, F. Van Bambeke Unité de pharmacologie cellulaire et moléculaire, Université catholique de Louvain, Brussels, Belgium *Institut de chimie des substances naturelles, CNRS, Gif sur Yvette, France Mailing address : P.M. Tulkens, Pharmacologie cellulaire et moléculaire, UCL Bat. Van Helmont, 7370 Av. E. Mounier,1200 Brussels, Belgium, [email protected]
منابع مشابه
Modulation of the expression of ABC transporters in murine (J774) macrophages exposed to large concentrations of the fluoroquinolone antibiotic moxifloxacin.
Long-term exposure to pharmacological agents can select for cells that overexpress efflux transporters. We previously showed that mouse J774 macrophages cultivated for a prolonged period of time with toxic concentrations of the fluoroquinolone ciprofloxacin overexpress the efflux transporter Mrp4 and display a reduced accumulation of this antibiotic, but no change in the accumulation of moxiflo...
متن کاملCooperation between prokaryotic (Lde) and eukaryotic (MRP) efflux transporters in J774 macrophages infected with Listeria monocytogenes: studies with ciprofloxacin and moxifloxacin.
Antibiotic efflux is observed in both eukaryotic and prokaryotic cells, modulating accumulation and resistance. The present study examines whether eukaryotic and prokaryotic fluoroquinolone transporters can cooperate in the context of an intracellular infection. We have used (i) J774 macrophages (comparing a ciprofloxacin-resistant cell line overexpressing an MRP-like transporter with wild-type...
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Probing fluoroquinolones/lipid interactions at the molecular level represents an important challenge in both membrane biophysics and pharmaceutical research. As the pharmacological target of these antibiotics is intracellular, they must pass across the bacterial membranes. Likewise, fluoroquinolones enter eukaryotic cells, which imply their ability of interacting with lipids membranes. In this ...
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Cystic fibrosis (CF) is associated with severe neutrophilic airway inflammation. We showed that moxifloxacin (MXF) inhibits IL-8 and MAPK activation in monocytic and respiratory epithelial cells. Azithromycin (AZM) and ciprofloxacin (CIP) are used clinically in CF. Thus we now examined effects of MXF, CIP, and AZM directly on CF cells. IB3, a CF bronchial cell line, and corrected C38 cells were...
متن کاملGemfibrozil enhances the listeriacidal effects of fluoroquinolone antibiotics in J774 macrophages
J774 macrophage-like cells express organic anion transporters that promote the efflux of fluoroquinolone antibiotics such as norfloxacin (NFX) from these cells. Gemfibrozil (GFZ) blocks organic anion transport in J774 cells, thereby facilitating the intracellular accumulation of NFX (Cao, C., H.C. Neu, and S.C. Silverstein. 1991. J. Cell Biol. 115:467a [Abstr.]). To determine whether GFZ enhanc...
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تاریخ انتشار 2009